منابع مشابه
On Novel Anti-viral HIV Gene Editing Platforms
Developing a new drug entity from drafting its structure to market launching is a complex process which can take many years and cost millions of dollars. It may also take many years to grow an idea of supporting evidence before selecting a pharmacological target for a costly drug discovery programme. This reflects a massive investment in terms of time, money, human and other potential resources...
متن کاملTALEN‐mediated gene editing of the thrombospondin‐1 locus in axolotl
Loss-of-function genetics provides strong evidence for a gene's function in a wild-type context. In many model systems, this approach has been invaluable for discovering the function of genes in diverse biological processes. Axolotls are urodele amphibians (salamanders) with astonishing regenerative abilities, capable of regenerating entire limbs, portions of the tail (including spinal cord), h...
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Using a TALEN-mediated gene-editing approach, we have previously described a process for the large-scale manufacturing of "off-the-shelf" CAR T cells from third-party donor T cells by disrupting the gene encoding TCRα constant chain (TRAC). Taking advantage of a previously described strategy to control TALEN targeting based on the exclusion capacities of non-conventional RVDs, we have developed...
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Precise studies of plant, animal and human genomes enable remarkable opportunities of obtained data application in biotechnology and medicine. However, knowing nucleotide sequences isn't enough for understanding of particular genomic elements functional relationship and their role in phenotype formation and disease pathogenesis. In post-genomic era methods allowing genomic DNA sequences manipul...
متن کاملBacterial Delivery of TALEN Proteins for Human Genome Editing
Transcription Activator-Like Effector Nucleases (TALENs) are a novel class of sequence-specific nucleases that have recently gained prominence for its ease of production and high efficiency in genome editing. A TALEN pair recognizes specific DNA sequences and introduce double-strand break in the target site, triggering non-homologous end joining and homologous recombination. Current methods of ...
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ژورنال
عنوان ژورنال: Human Genetics
سال: 2016
ISSN: 0340-6717,1432-1203
DOI: 10.1007/s00439-016-1678-2